Table 2.
ES lines from epiblast of delayed-implanting blastocysts
Type of feeders | Strain | No. of epiblasts | No. giving ES lines (%) |
---|---|---|---|
Explanted onto STO feeders | 129 | 17 | 17 (100) |
PO | 7 | 0 | |
[PO × ROSA26]F1 | 4 | 0 | |
C57BL/6 | 3 | 1 (33) | |
Explanted onto primary embryonic fibroblast feeders | 129 | 6 | 6 (100) |
PO | 9 | 5 (56) | |
[PO × ROSA26]F1 | 4 | 0 | |
CBA/Ca | 61 | 13 (21) | |
Explanted onto primary embryonic fibroblast feeders + LIF | [PO × ROSA26]F1 | 8 | 4 (50) |
CBA/Ca | 18 | 10 (56) |