Fig. 5.

Overexpression of AGS3 disrupts the localization of two endogenous TGN cargo proteins without affecting those of cis-Golgi/cis-Golgi network and medial-Golgi proteins. (A) COS7 cells overexpressing AGS3 were counted (n > 200, only cells highly overexpressing AG53 were counted) based on whether the staining of Golgi marker proteins had predominantly perinuclear or diffuse staining. (B) The localization of p115 is not significantly different in those cells highly overexpressing AGS3, whereas localization of the TGN cargo proteins TGN46 and CD-MPR are dramatically altered in cells with high levels of AGS3. Cells overexpressing and not overexpressing AGS3 were captured in the same field for comparison. (Scale bars: 20 μm.)