Fig. 2.

Constitutive surface expression of the NKG2D ligand RAE-1 on hepatocytes is up-regulated specifically on HBV-Env-expressing hepatocytes, before adoptive transfer. (A) Surface expression of RAE-1 on hepatocytes from HBV-Env⁺ RAG^−/− (solid line) and RAG^−/− (dotted line) mice. Shaded histograms depict staining using the appropriate isotype-matched control Ig. (B) RAE-1 mRNA expression in hepatocytes from HBV-Env⁺ RAG^−/− (black bar) and RAG^−/− (white bar) mice and from intrahepatic (IH) immune cells and splenocytes from RAG^−/− mice in comparison with HPRT expression. All data are representative of at least three independent experiments.