Culture and viable counting of microorganisms on chips. Dilution series of microorganisms plated on a series of 20 × 20-μm format chips. The number of deposited cfu per chip was calculated from the fraction of compartments supporting growth, as scored by microscopy, with the numbers corrected for inoculation of compartments with multiple cfu. Open triangles, E. coli; filled squares, C. albicans; filled diamonds, L. plantarum. Error bars show SD with lower limits not shown at lowest dilution factor because of log10 transformation of the data.