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. Author manuscript; available in PMC: 2008 Sep 21.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Jul 23;361(2):555–560. doi: 10.1016/j.bbrc.2007.07.063

Fig. 2.

Fig. 2

The pH profile and the effects of metal ions, EDTA, and DTT on the α2,3-sialyltransferase activity of Hd0053 by quantitative HPLC analysis. (A) The pH profile. Activity was measured at indicated pH at 37 °C for 60 min. Buffers (200 mM) used were: MES, pH 5.0−6.0; Hepes, pH 7.0; Tris–HCl, pH 7.5−9.0; and CAPS, pH 10−11. (B) Effects of metal ions, EDTA, and DTT. The activity was determined in Tris-HCl buffer (100 mM, pH 8.5) at 37 °C for 60 min.