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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Chem Biol. 2007 Jul;14(7):835–846. doi: 10.1016/j.chembiol.2007.06.008

Figure 2.

Figure 2

Autoradiogram of TaVC activity. TaVC was individually combined with phosphopantetheinylated TaB, TaE, Ta1-T6, Ta1-T7 and Ta1-T8 in the presence of radiolabeled acyl-CoA thioesters and subjected to denaturing gel electrophoresis. The phosphopantetheinyl transferase Sfp remaining from in situ generation of the holo-thiolation domains is visible. (Left) Coomassie-stained gel; molecular weight standards are 15 kD, 21 kD, 28 kD, 41 kD, and 58 kD. (Right) Autoradiogram; molecular weight standards are 15 kD, 21 kD, 28 kD, and 45 kD. Ac, acetyl; Prop, propionyl; Mal, malonyl; Memal, methylmalonyl.