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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Chem Biol. 2007 Jul;14(7):835–846. doi: 10.1016/j.chembiol.2007.06.008

Figure 3.

Figure 3

Radioassay of TaK activity. 1,3-14C-Mal-S-TaB (filled squares), 1,3-14C-Mal-S-TaE (open squares), and 1,3-14C-Memal-S-TaE (open circles) were incubated with TaK. At several time points, reaction aliquots were withdrawn and quenched by addition of trichloroacetic acid, and residual protein-linked radiolabel was measured by liquid scintillation counting. Loss of protein-linked radiolabel is associated with generation of 1-14C-Ac-S-TaB from 1,3-14C-Mal-S-TaB with concomitant evolution of 14CO2. Each point represents the average of three trials.