Table 1.

Sedimentation coefficients and molecular mass of PDO as determined by Analytical ultracentrifugation.

Conditionsa	Detection method	Loading concentration (signal units)	s20,W, S (± 0.2 S)	Mass, kDa (± 10 kDa)	PDO oligomer sizeb
Low salt	Interference	3.40	11.78	363	7.3
Low salt	Absorbance at 405 nm	0.12	11.99	372	7.6
Low salt	Absorbance at 455 nm	0.16	11.99	387	7.9
High salt	Interference	0.39	11.50	318	6.5
High salt	Absorbance at 280 nm	0.34	11.81	283	5.8
High salt	Interference	0.92	11.37	301	6.1
High salt	Absorbance at 280 nm	0.72	11.66	284	5.8

^aBuffer for low salt was 20 mM KPO₄, pH 6.9; for high salt, 50 mM KPO₄, 0.5 MKCl, pH 7. Buffers contained 5 mM phthalate.

^bBased on monomer mass of 49 kDa.