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. Author manuscript; available in PMC: 2007 Nov 21.
Published in final edited form as: J Biol Chem. 2007 Feb 5;282(23):16718–16728. doi: 10.1074/jbc.M700647200

Figure 7. Effects of Fenretinide on Cell Growth and pRB.

Figure 7

(A) The effects of low dose 4-HPR treatment (1 and 2.5 μM) on cell growth were determined via MTT assay as described in “Experimental Procedures.” The figures presented are representative of at least two independent experiments performed in triplicate. The error bars represent the standard deviations, and when not seen, they are smaller than the thickness of the lines on the graphs. (B) Protein levels of pRb, β-actin, and DEGS-1 were detected by Western blotting. Cells were pretreated for ~18 h with either 10 nM OA (B, center panel) or 10 nM TMY (C, right panel) prior to 2.5 μM 4-HPR treatment. Total cell lysates were prepared 48 h after 4-HPR treatment. Equal amounts of proteins (30 μg) were run on 10% SDS-PAGE and blotted onto an Immobilon membrane as described in “Experimental Procedures.” β-actin was probed with to verify equal loading of proteins per lane. The figures presented are representative of at least three independent experiments.