Skip to main content
. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2007 May 24;106(1-5):111–118. doi: 10.1016/j.jsbmb.2007.05.009

Figure 3. Expression of estrogen and progesterone receptors in HER-2/neu × aromatase double transgenic mice and parental strains along with wild type control.

Figure 3

Quantitative real-time RT-PCR analysis was carried out to determine the mRNA levels of ERα and β along with progesterone receptor (PR) as described in Materials and Methods and figure 2. Inset: Figure shows a representative western blot analysis data of ERα and β and PR protein levels in HER-2/neu double transgenic mice along with parental types and wild type control. Western blot analysis of the protein extracted from different frozen mammary tissues using antibodies against ERα and β and PR along with β-actin (loading control). Each lane contains 60μg of protein.