Figure 5. Expression of cyclin D1 and pRb and ppRb in HER-2/neu × aromatase double transgenic mice and parental strains along with wild type control.
Western blot analysis of the protein extracted from different frozen mammary tissues using antibodies against cyclin D1, unphosphorylated (pRb) and phosphorylated (phos-pRb) along with β-actin (loading control). Each lane contains 75μg of protein.