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. Author manuscript; available in PMC: 2007 Dec 21.
Published in final edited form as: Oncogene. 2007 Jan 8;26(29):4295–4305. doi: 10.1038/sj.onc.1210194

Figure 4.

Figure 4

c-Myc and c-Jun, but not p53, are important for RhoAQ63L-dependent cell transformation. (a) NIH3T3 cells were transfected with the transactivation reporter plasmids (top) in the presence (RhoAQ63L) or absence (Mock) of the mammalian expression vector encoding RhoAQ63L and luciferase activities quantified as indicated in Supplementary Materials and methods . Values are expressed as fold change variations with respect to the appropriate control sample (that was given an arbitrary value of 1) and are the mean±s.d. of four independent experiments, each of them performed in triplicate. pP2luc, pM4luc, AP1luc and p53luc are reporter plasmids containing the c-myc promoter, c-Myc binding sites, AP1 binding sites and p53 binding sites, respectively. (b, c) Primary focus formation assays showing the transforming activity of RhoAQ63L when expressed alone or in combination of the indicated proteins. DNM, dominant-negative mutant.