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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Neurochem Res. 2007 Jun 13;32(12):2080–2093. doi: 10.1007/s11064-007-9368-z

Fig. 6. Caspase-3 activity in injured spinal cords.

Fig. 6

(A) Quantitative estimation of the number of caspase-positive cells in each section. Results are from three independent sections between 1 and 2mm caudal to the injury epicenter (n = 3). (B) Caspase-3 enzyme activity was assayed in tissue lysates of spinal cord. Equal amounts of protein (50 μg) were assayed for caspase-3 activity for 30 min in an ELISA plate reader, measuring absorption at 405 nm. Purified caspase-3 enzyme was used as a positive control. The injured tissues show maximum enzymatic activity when compared to the treated tissues, which show activity similar to that of the control groups. Inh. = Ac-DEVD-CHO inhibitor. (Error bars indicate SEM. * Significant at p <0.05). The experiment is repeated twice with triplicates (n = 3).