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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Neurochem Res. 2007 Jun 13;32(12):2080–2093. doi: 10.1007/s11064-007-9368-z

Fig. 7. Immunoblot analysis of caspase-3-mediated apoptotic pathway in spinal cord sections.

Fig. 7

Equal amounts of protein (40 μg) were loaded onto 10%-14% gels and transferred onto nylon membranes, which were then probed with respective antibodies. The blots were stripped and reprobed with GAPDH to assess protein levels. A and C shows the Fas and caspase-3 mediated apoptotic pathway proteins with respect to GAPDH; B and D are their quantitative estimations, respectively. Inhibition of the caspase-3-mediated apoptotic pathway by rMSC shows downregulation of Fas and caspases, upregulation of FLIP, XIAP, and inhibition of PARP cleavage. Antisera for caspase-8 and caspase-3 recognize cleaved fragments also. Each blot is representative of experiments performed in duplicate with each sample (n = 3). Error bars indicate SEM. * Significant at p <0.05.