Table 1.

Translesion synthesis within DNA sequences containing AAF adducts

Measurement	Wild type		ΔumuDC
	SOS−	SOS+	SOS−	SOS+
TLS at -GGGAAF-
Nonslipped TLS*	2.34  ± 1.2	22.8  ± 3.7	2.65  ± 1.48	2.07  ± 0.95
(Number of white clones)	(454)	(1,668)	(1,555)	(189)
Slipped TLS†	0.10  ± 0.07	3.08  ± 0.31	0.072  ± 0.003	1.37  ± 0.08
(Number of blue clones)	(20)	(226)	(42)	(125)
Survival	2.44	25.88	2.72	3.44
Mutation frequency,‡ %	4.1	11.9	2.6	40
TLS at -GGCGAAFCC-
Nonslipped TLS*	3.06  ± 1.48	16.7  ± 1.0	2.19  ± 0.45	2.31  ± 0.78
(Number of white clones)	(329)	(1,197)	(251)	(39)
Slipped TLS†	1.03  ± 0.34	34.5  ± 0.84	2.70  ± 0.78	54.3  ± 1.2
(Number of blue clones)	(111)	(2,464)	(309)	(913)
Survival	4.09	51.2	4.89	56.6
Mutation frequency,‡ %	25.2	67.4	55.2	96

Nonmodified vectors form only white colonies. The total number of blue and white colonies that have been scored to obtain the TLS values are indicated in parenthesis. The average value of three independent experiments and the standard deviation are indicated. Survival is the sum of nonslipped and slipped TLS. 

^*Nonslipped TLS is the number of white colonies per ng of modified vector divided by the number of (white) colonies per ng formed by the corresponding nonmodified vector, expressed as a percentage. 

^†Slipped TLS is the number of blue colonies per ng of modified vector divided by the number of (white) colonies per ng formed by the corresponding nonmodified vector, expressed as a percentage. 

^‡The mutation frequency is the percent of slipped TLS divided by total TLS. It represents the elongation error-rate of the replication machinery per TLS event.