Table 2.
The effect of T- or NK cell-depletion in mice bearing B16 melanoma on the antitumor activity of BAT
| BAT treatment | Number of metastases
|
|||
|---|---|---|---|---|
| Exp. 1
|
Exp. 2
|
|||
| − | + | − | + | |
| Nondepleted | 113 ± 80 | 0.5 ± 1 | >200 | 0 |
| T cells depleted | >200 | 56 ± 9 | >200 | 37 ± 23 |
| NK cells depleted | >200 | >200 | >200 | 160 ± 60 |
C57BL mice were injected i.p. with either anti-CD3 (100 μg) or anti-asialo GM1 (35 μg) 1 day prior to B16 melanoma injection and on days 7, 14, and 21 days posttumor inoculation. Experiment 1: Mice injected with anti-CD3 or anti-asialoGM1 on day 12 and 22 posttumor inoculation. Experiment 2: Five mice were used in each group. Animals were sacrificed on day 24 posttumor injection and the numbers of metastases were scored. Results are expressed as the means ± SD.