Fig. 4.

To examine the purity of oligodendrocytes, cells were double-immunolabeled with GalC and either CD11b, GFAP or MAP-2 and observed under a confocal laser-scanning microscope (a). Differentiated oligodendrocytes were treated with 30 ng/ml of TNF-α and after 24 h of treatment, cells were immunostained with antibodies against MOG (b). Human primary oligodendrocytes were incubated with 300 μM of H₂O₂ for 6 h in serum free media followed by TUNEL (c). Results are representative of three separate experiments