Table I.

Sequences of primers used to introduce SNPs

Primer name	Sequencea	Amino Acid Change
191A-for	5’-CATTGTAAGAAGAAACCAGGGTGGGTGGTGTCTC-3’	R64Q
191A-rev	5’-GAGACACCACCCACCCTGGTTTCTTCTTACAATG-3’
282C-for	5’-GGAGGGTATTTTTATATCCCTCCAGTTAAC-3’	None
282C-rev	5’-GTTAACTGGAGGGATATAAAAATACCCTCC-3’
341C-for	5’-CTCCTGCAGGTGACCACTGACGGCAGGAATTAC-3’	I114T
341C-rev	5’-GTAATTCCTGCCGTCAGTGGTCACCTGCAGGAG-3’
364A-for	5’-GCAGGAATTACATTGTCAATGCTGGGTCTGGAAGC-3’	D122N
364A-rev	5’-GCTTCCAGACCCAGCATTGACAATGTAATTCCTGC-3’
411T-for	5’-GTGGCAGCCTCTAGAATTTATTTCTGGGAAGGATCAG-3’	L137F
411T-rev	5’-CTGATCCTTCCCAGAAATAAATTCTAGAGGCTGCCAC-3’
481T-for	5’-GAGAGAGGAATCTGGTACTTGGACCAAATCAGGAGAGAG-3’	None
481T-rev	5’-CTCTCTCCTGATTTGGTCCAAGTACCAGATTCCTCTCTC-3’
499A-for	5’-GGACCAAATCAGGAGAAAGCAGTATATTAC-3’	E167K
499A-rev	5’-GTAATATACTGCTTTCTCCTGATTTGGTCC-3’
590A-for	5’-TTACGCTTGAACCTCAAACAATTGAAGATTTTGAG-3’	R197Q
590A-rev	5’-CTCAAAATCTTCAATTGTTTGAGGTTCAAGCGTAA-3’
803G-for	5’-GAGGTTGAAGAAGTGCTGAGAAATATATTTAAGATTTCCTTGGGG-3	K268R
803G-rev	5’-CCCCAAGGAAATCTTAAATATATTTCTCAGCACTTCTTCAACCTC-3’
857A-for	5’-CCAAACCTGGTGATGAATCCCTTACTATTTAG-3’	G286E
857A-rev	5’-CTAAATAGTAAGGGATTCATCACCAGGTTTGG-3’

^aThe mismatched nucleotide(s) used for mutagenesis is underlined for each primer.