Figure 3.

Comparison of presentation by M1.DR1, M1.DR1.Ii, and DR1.B-LCL to two alloreactive T cell clones raised against PBMC. Titrations of mitomycin-C-treated M1.DR1 (•), M1.DR1.Ii (○), or irradiated DR1.B-LCL (□) were used to stimulate 10⁴ alloreactive T cells. After 48 hr, 1 μCi of [³H]thymidine was added to each well. The cultures were harvested 16 hr later and radioactivity was measured by liquid scintillation spectroscopy. Results are expressed as mean Δ cpm from triplicate cultures. SEM were all less than 10%.