Table 3.
Antibodies used for staining and biochemistry
| Antibody | Specificity | Isotype | Nature | Source (Ref.) |
|---|---|---|---|---|
| L243 | HLA-DRα | IgG2a | Culture supernatant | ATCC* (24) |
| 16.23 | HLA-DR3 | IgG3 | Ascites fluid | † (25) |
| VICY1 | Ii | IgG1 | Ascites fluid | ‡ (26) |
| CerCLIP.1 | CLIP | IgG1 | Ascites fluid | § (9) |
| 5C1 | HLA-DMα | IgG | Culture supernatant | § (27) |
| FITC anti- | Mouse IgG | Sheep antiserum | Sigma |
FITC, fluorescein isothiocyanate.
*
American Type Culture Collection, Rockville, MD.
†
Gift from J. Trowsdale (Imperial Cancer Research Fund, London).
‡
Gift from W. Knapp, (University of Vienna, Vienna, Austria).
§
Gift from P. Cresswell.