Figure 1.

Schematic representation of the genome of 30BRz (derivative of TMV) and the cloning strategy: the 126- and 183-kDa proteins are required for the TMV replication, 30-kDa protein is the viral movement protein, and CP is viral CP. The arrow under TMV CP SP indicates the subgenomic promoter of TMV CP. The two ellipsoids indicates peptides fused to the AIMV CP. Rz, ribozyme for self-cleavage. pBRzCPMNV3 and pBRzCPDrg24 are viruses engineered to express antigenic peptides representing V3 loop of HIV-1 named MNV3 or chimeric epitope of rabies virus named Drg24, respectively. The amino acid sequence of inserted region of each peptide is shown under the linear map of plasmid.