Figure 2.

Western blot showing accumulation of recombinant AIMV CP fused to MNV3 of HIV-1 or Drg24 of rabies virus in locally (L) or systemically (S) infected tobacco leaves. The tobacco leaves were inoculated with transcripts of recombinant virus. Proteins were separated by electrophoresis through a SDS/13% polyacrylamide gel and bound with mAbs for the AIMV CP (A, lanes 2 and 3; B, lanes 1 and 2), for the linear epitope (G5-24) of rabies virus glycoprotein (C, lanes 2 and 3), or for the V3 loop of the HIV-1 (D, lanes 2 and 3). Wild-type AIMV CP (24 kDa) bound only with antibodies against AIMV CP (A, lane 1, positive control) and did not bind with antibodies against fusion peptides (C and D, lanes 4; negative control). Each protein is indicated above the lane.