Figure 4.

Serum antibody response of mice immunized intraperitoneally with CPDrg24 measured by ELISA (a and b) on a plates coated with inactivated rabies virus (ERA) and neutralizing activity of these antibodies. Each graph represents the average of the values calculated for individual mice. 30B/AIMV indicates the sera from the mice immunized with control (wild-type virus lacking insert). (a) The data when the antigen was administered without CFA; whereas b represents the data when CFA was incorporated. The curves reflect average ELISA data at different dilutions of sera. Graph c shows neutralization of the CVS-11 strain rabies virus by sera from mice immunized with recombinant CPDrg24, 30B/AIMV, or preimmune. The curves represent neutralizing titers of sera after seven immunizations. (c) Average data with SD from eight mice that had neutralizing serum antibodies.