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Localization of apoptin and staining of the chromatin in three normal human cell types, transiently transfected with pCMV-fs. Apoptin was stained with anti-apoptin mAb 85.1 (A, C, and E) and the DNA was stained with DAPI (B and D) or PI (F), in representations of identical cells: (A and B) HSMC, (C and D) HUVEC, and (E and F) T cells. Cells were fixed 5 days after transfection and analyzed by indirect immunofluorescence. (Original magnification: A and B, ×630; C–F, ×1,000.)
