Figure 9.

Maintenance of IGF-IR transcript levels in C6(t1) cells transfected with antisense or triplex effector sequences against IGF-I. Total RNA (15 μg per lane) from vector-transfected C6(t1) rat glioblastoma cells (lane 1), antisense to IGF-I-transfected cells (lane 2), six different cell clones transfected with polypurine triplex sequences against IGF-I (lanes 3–8), or a cell clone transfected with polypyrimidine triple-helix sequences against IGF-I (lane 9) was analyzed using an IGF-IR cDNA as a hybridization probe. Rehybridizations of the same filter were performed with cDNA probes for IGF-I (B) or chicken β-actin (C).