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. 1997 May 27;94(11):5866–5871. doi: 10.1073/pnas.94.11.5866

Figure 1.

Figure 1

FACS analysis of surface EpoR expression and induction of Cɛ germline transcripts in BJAB transfectants. (A) Surface EpoR expression: BJAB cells transfected with pcDNA3 vector alone, EpoR, or EpoR/IL-4Rα constructs were subjected to G418 selection and fluorescence activated cell sorting, then analyzed for murine EpoR surface expression by FACS. Background fluorescence was determined by staining with normal rabbit serum (—). Filled fields show staining with rabbit anti-EpoR antiserum. Similar results were obtained in two independent transfections. (B) Expression of Cɛ germline transcripts: Transfectants were left unstimulated (None), or stimulated with Epo (50 units/ml) or IL-4 (200 units/ml) for five days then assessed for expression of Cɛ germline transcripts by Northern blot analysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was used as standard for equal loading. Similar results were obtained in three independent experiments.