Figure 2.

FACS analysis of surface CD8 expression and induction of Cɛ germline transcripts in BJAB transfectants. (A) Surface CD8 expression: BJAB cells transfected with pCEP4 vector alone, CD8, or CD8/IL-4Rα constructs and selected in hygromycin were examined 13 days after transfection for surface expression of human CD8 by FACS. Background fluorescence was determined by staining with isotype control antibody (—). Filled fields show staining with anti-CD8 mAb. Similar results were obtained in two independent transfections. (B) Expression of Cɛ germline transcripts: expression of Cɛ germline transcripts and of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were examined by Northern blot analysis on the indicated days after transfection. Similar results were obtained in three independent experiments.