Figure 4.

Kinetic parameters of ADPR cyclase from E₂-treated rats (•) and from the control rats (□) evaluated by the Lineweaver–Burk double-reciprocal plot. ADPR cyclase was measured with NGD as a substrate in concentration range 10–400 mM NGD. Km_NGD from E₂-treated rats (30 μM) was not different from Km_NGD of controls (33 μM). V_MAX value from E₂-treated uterine extract (1.76 nmol cGDPR/min per mg protein) was >3 times higher than V_MAX in extracts from controls (0.52 nmol cGDPR/min per mg protein).