Fig. 5.

Comparison of ligand-dependent expression of the β-galactosidase reporter gene mediated by the wild-type and redesigned RBPs. Error bars indicate the standard deviation of three independent measurements. The reported β-galactosidase activity is normalized relative to cell cultures grown in the absence of ligand. There is no Zn²⁺ dependence of β-galactosidase expression by the wild-type RBP construct. Shaded hatched bars, ribose; unshaded hatched bars, Zn²⁺.