. 2003 Sep 19;100(20):11255–11260. doi: 10.1073/pnas.2032284100

Table 1. Mutations constructed in RBP to introduce Zn²⁺-binding sites.

	Mutations^*
Design	PCS	SCS^†
A	Phe-15_IGlu	Arg-90_IAsp(89)
	Asp-89_IHis_ε	Ser-103_HGln(235)
	Asp-215_IIGlu	Arg-141_IIGln
	Gln-235_HHis_δ	Phe-214_IISer
B1 and B2	Asp-89_IHis_ε	Arg-90_ISer
	Thr-135_IIGlu	Asn-105_IITyr(89)
	Ala-137_IIGlu/His_δ^‡	Arg-141_IIGln(137^§)
	Asn-190_IIHis_ε	Phe-164_IIThr(190)

Subscripts indicate the region in which the residue is located (Fig. 1). I, N-terminal domain; II, C-terminal domain; H, hinge; δ and ε, imidazole nitrogen that coordinates Zn²⁺.

^†

Numbers in parentheses indicate the PCS histidine to which the SCS residue is hydrogen bonded.

^‡

Glu in B1, His in B2.

^§

Gln in both designs, hydrogen bonding in B2 only.

Table 1. Mutations constructed in RBP to introduce Zn2+-binding sites.

Table 1. Mutations constructed in RBP to introduce Zn²⁺-binding sites.