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. 1997 May 27;94(11):5882–5887. doi: 10.1073/pnas.94.11.5882

Table 2.

Siderophore production by wild-type 518, urbs1 gene disruption mutant UMC015, and urbs1 NTG mutants carrying a single amino acid mutation in the C-terminal finger domain under low and high iron conditions

Strain Low iron High iron LI/HI
518 0.12  ±  0.08* 0.01  ±  0.005 12
UMC015 0.16  ±  0.02 0.05  ±  0.01 3.2
UMC002-1 (urbs1-1) 0.14  ±  0.08 0.06  ±  0.02 2.3
UMC005-1 (urbs1-2) 0.16  ±  0.01 0.01  ±  0.00 16
UMC007-1 (urbs1-3) 0.14  ±  0.02 0.04  ±  0.01 3.5

Cells were grown for 48 h at 28°C in low iron medium with or without 10 μM FeSO4. Siderophore production was determined by the ferric perchlorate assay as described. Equal volumes of cell free supernatant and assay solution were mixed and the adsorbance read at 495 nm. Data were normalized to cell growth using an adsorbance at 600 nm equal to 1.0. LI, low iron; HI, high iron. 

*

Means of three replications ± SE.