Table 3.
Siderophore production by urbs1 gene disruption mutant UMC015 transformed with plasmid containing epitope-tagged wild-type urbs1 (pAN15) and in vitro mutated urbs1 alleles (pAKR350L and pAKR494L) under low and high iron conditions
| Strain | Low iron | High iron | LI/HI |
|---|---|---|---|
| UMC015/pAN15 | 0.12 ± 0.04* | 0.02 ± 0.00 | 6 |
| UMC015/pAKR350L | 0.12 ± 0.01 | 0.02 ± 0.00 | 6 |
| UMC015/pAKR494L | 0.17 ± 0.02 | 0.13 ± 0.02 | 1.3 |
Cells were grown for 48 h at 28°C in low iron medium with or without 10 μM FeSO4. Siderophore production was determined by the ferric perchlorate assay as described. Values were normalized to a cell growth of OD600 = 1.0. LI, low iron; HI, high iron.
*
Means of three replications ± SE.