Fig. 2.
(a) Kinetic scheme for multisite hydrolysis of ATP by F1-ATPase. S represents an ATPase with the βTP site occupied, because it is always occupied by ATP under the condition considered (see text). S(βE,T) has βTP and βE both occupied by ATP; S(βDP,T) has βTP and βDP both occupied by ATP, whereas S(βDP,D) has βTP and βDP occupied by ATP and ADP, respectively. All three catalytic sites of S(βE,T;βDP,T) and S(βE,T;βDP,D) are occupied. The symbol T represents an ATP and the D stands for an ADP+Pi. The ki values are rate constants (see text and legend of Fig. 3). (b) Schematic diagram for the ATP hydrolysis reaction, where only the species important for ATP hydrolysis (see text) are shown.