Table 1. Localized transcripts.

	RNA localization
Gene	Full	Coding	she2Δ	Cell cycle regulation	Predicted function	Protein localization	Tag position
ASH1	Yes	Yes	No	M	Transcription	Bud nucleus (51-53)	N.C
BRO1	Yes	Yes	No	None	Stress transduction	Punctae on vacuole	C
CLB2	Yes	Yes	No	M	Cyclin B	Nuclei, spindle poles	C
CPS1	Yes	Yes	No	None	Carboxypeptidase	Cytoplasmic punctae	N
DNM1	Yes	Yes	No	S	Mitochondrial fission	Mitochondrial periphery (18, 54)	C
EGT2	Yes	Partial	No	M	Cellulase	Membranes, large-bud enriched	C
ERG2	Yes	Yes	No	M	Sterol isomerase	Endoplasmic reticulum	N
IST2	Yes	Yes	No	None	Tranporter	Bud plasma membrane (13)	N
MID2	Yes	Yes	No	None	Membrane receptor	Cell periphery, mother-bud junction	C
MMR1	Yes	Yes	No	M	Unknown	Bud sites and tips, mother-bud junction	N
SRL1	Yes	Yes	No	G1	Unknown	Periphery of small buds	C
TPO1	Yes	Yes	No	M	Polyamine transport	Bud plasma membrane	N
WSC2	Yes	Yes	No	S	Membrane receptor	Membranes, bud-enriched	C
YGR046W	Yes	Yes	No	None	Unknown	Mitochondria	N
YJL051C	Yes	Yes	No	M	Unknown	Membranes, bud-enriched	N
YLR434C	Yes	Yes	No	None	Unknown	Mitochondria	N
YML072C	Yes	Yes	No	G2	Unknown	Membranes, bud-enriched	C
YMR171C	Yes	Yes	No	None	Unknown	Endoplasmic reticulum	N
YNL087W	Yes	Yes	No	None	Unknown	Membranes, bud-enriched	C
KSS1	Partial	ND	No	None	Mitogen-activated protein kinase	ND	ND
LCB1	Partial	Partial	No	None	Endoplasmic reticulum, lipid synthesis	Endoplasmic reticulum	C
MET4	Partial	Partial	No	None	Transcription	Nuclei	C
MTL1	Weak	ND	No	None	MID2-like	ND	ND
YPL066C	Weak	ND	No	None	Unknown	ND	ND

List of She-localized mRNAs. Also indicated are whether each transcript is cell cycle-regulated and its peak stage (49, 50), the predicted or known function, and protein localization of each. Proteins that are asymmetrically enriched in the bud are indicated by boldface. The full list of transcripts assayed for localization as well as their percentile rankings from microarray analysis can be viewed in Table 2. Full array results and additional methodology can be viewed in Supporting Materials and Methods and at http://valelab.ucsf.edu/~shepard/methods.html or http://genome-www5.stanford.edu/MicroArray/SMD/.C, carboxyl terminus; N, amino terminus. Yes, ≥90% bud localization; Partial, 50-60% localization; Weak, 15-30% localization; No, unlocalized (<5% localization; ND, not determined).