Fig. 1.

In vivo angiogenesis assay in matrigel plugs. Mice bearing matrigel plugs impregnated with bFGF were treated with daily i.p. injections of 1 mg of anastellin (anast), PBS, or a control fragment homologous to anastellin from the 11th type III domain of fibronectin for 10 days. Angiogenesis was evaluated by counting the number of blood vessels in the plugs (A) or by measuring the hemoglobin content in duplicate plugs (B). Combined results from three independent experiments, with three or four mice in each test group, are shown. The P values show the significance level of the differences observed between the indicated test groups. NS, not significant.