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. 2003 Sep 18;100(20):11469–11473. doi: 10.1073/pnas.1634991100

Fig. 2.

Fig. 2.

Ci-Nkx expression. The top row displays diagrams of representative stages as in Fig. 1. (Right Upper) The diagram portrays a juvenile ≈36 h after metamorphosis, with the resorbed tail muscle in red. The anterior is to the left in all diagrams and pictures. (A–E) Ciona larvae stained with antisense RNA probes against Ci-Nkx (ci0100140552, clone ID no.GC29k02). (A–C) Side views of a tailbud head (A), larval head (B), and a juvenile (C) ≈36 h after metamorphosis. Note staining of the differentiating heart (arrowhead). Contractile activity in the heart can first be detected ≈60 h after metamorphosis. Also shown are ventral views of a tailbud head (D) and a larval head (E). Note staining of the TVCs in A and D (arrow). We have also included a side view of an ≈36-h-old juvenile stained with Ci-TnI (F). Note staining of the siphon muscles (arrow), along with the heart (arrowhead).