Figure 1.

Point mutation of NRSE in the promoter of the nAChR β2-subunit gene changes the pattern of β-gal expression in transgenic mice. (a–d) Whole-mount coloration of E13.5 transgenic embryos carrying the wild-type promoter (a and c) and with point mutation in NRSE (b and d). (e and f) Detection of the β-gal activity and the glial fibrillary acidic protein immunoreactivity in the adult mutated transgenic brain. (e) Dorsal part of the hippocampus. (f) Dorso-medial part of the cortex, showing the absence of β-gal expression in the molecular layer (arrow). og, orthosympathetic ganglia; drg, dorsal root ganglia; Co, colliculus; Th, thalamus; Cx, cortex; Hip, hippocampus; cc, corpus callosum.