Fig. 3.

(A) The proximal promoter sequence of the human β₁ sGC gene. Circumscribed boxes indicate the cores of binding sites for transcriptional factors CBF, GFI1, Sp1, and NF1 that were deleted in reporter constructs and EMSA oligos. EMSA oligonucleotide probe sequences are underlined, and positions of the 5′ and 3′ ends of each oligonucleotide relative to the transcriptional start site are indicated. (B) Effects of core deletions for CBF, NF1, GFI1, and SP1 factors on activity of the p 0.8 construct. BE2 cells were transfected with the p 0.8 luciferase construct containing singular core deletions of CBF (pCAAT), NF1 (pNF1), and GFI1 (pGFI1) binding sites; double core deletions of CBF and GFI1 (pCAAT-GFI1) binding sites, of SP1 and NF1 (pSPNF) binding sites; and triple core deletion of SP1, NF1 and CBF (pCAAT-SPNF) binding sites. Activity was measured in relative light units per μg of protein lysates and expressed as the percentage of control p 0.8 plasmid activity. Results are means ± SD (n = 9).