Table 3. Canr rate of strains mutated in oxidative stress-response genes.
| Gene deleted | Rate (× 10-7)* | Wild-type function |
|---|---|---|
| Wild type | 3.4 | |
| cTPx II | 5.1 | Cytoplasmic thiol peroxidase isoform II |
| cTPx III | 5.6 | Cytoplasmic thiol peroxidase isoform III |
| mTPx | 5.3 | Mitochondrial thiol peroxidase isoform |
| nTPx | 6.4 | Nuclear thiol peroxidase isoform |
| SOD2 | 5.8 | Manganese superoxide dismutase |
| TRX1 | 4.5 | Thioredoxin 1 |
| TRX2 | 4.0 | Thioredoxin 2 |
| TRX3 | 3.3 | Mitochondrial thioredoxin |
| TRR2 | 4.2 | Mitochondrial thioredoxin reductase |
| GPX1 | 3.8 | Glutathione peroxidase |
| GPX2 | 3.8 | Glutathione peroxidase |
| HYR1 | 7.7 | Glutathione peroxidase |
| GSH1† | 0 | γ-Glutamylcysteine synthetase |
| GSH2 | 4.6 | Glutathione synthetase |
| CCP1 | 4.2 | Cytochrome c peroxidase |
| CTT1 | 4.5 | Catalase T |
| CTA1 | 3.7 | Catalase A |
| ZWF1 | 6.4 | Glucose-6-phosphate dehydrogenase |
| TKL1 | 3.4 | Transketolase |
| RPE1 | 2.7 | Ribulose-5-phosphate epimerase |
*
Average of two independent experiments, each with nine cultures. Mutations were in the BY4741 background.
†
No colonies grew on SC arginine-dropout plates containing 60 mg/liter canavanine.