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. 2003 Sep 3;100(20):11553–11558. doi: 10.1073/pnas.1833911100

Fig. 2.

Fig. 2.

(A) Comparison of the protein sequences of human (h) DYX1C1 and mouse (m) Dyx1c1. The SNPs found in this study are marked with arrowheads, and the three TPR domains are marked with asterisks. SNPs in the 5′-UTR are also shown. (B) RT-PCR from human multiple-tissue cDNA panels I and II (Clontech). Lanes: 1, phage λ/φX174 DMA size marker; 2, heart; 3, brain; 4, placenta; 5, lung; 6, liver; 7, skeletal muscle; 8, kidney; 9, pancreas; 10, spleen; 11, thymus; 12, prostate; 13, testis; 14, ovary; 15, small intestine; 16, colon; and 17, leukocytes. (C) DYX1C1 Northern blot showing a band of ≈2 kb. Lanes: 1, spleen; 2, thymus; 3, prostate; 4, testis; 5, ovary; 6, small intestine; 7, colon; 8, leukocytes; 9, brain; and 10, placenta. (D) Cellular localization of DYX1C1 protein. COS-1 cells transfected with DYX1C1-V5 fusion construct were stained with monoclonal mouse anti-V5 antibody and FITC-conjugated anti-mouse-IgG (green). 4′,6-Diamidino-2-phenylindole (DAPI)-stained nuclei are shown blue.