Table 1. Genotypic identity at MHC class I and II loci by cDNA sequencing.
% nucleotide sequence homology
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MHC class I loci
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MHC class II loci
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Strain | H2-K | H2-D | H2-Aα | H2-Aβ | H2-Eα | H2-Eβ |
H2b haplotype | ||||||
GenBank strain | C57BL/10 | C57BL/10 | 129 | NA | NA | B6 |
B6 | 100 | 100 | 100 | 100 | — | 100 |
BALB.B | 100 | 100 | 100 | 100 | — | 100 |
H2k haplotype | ||||||
GenBank strain | C3H | C3H | A/J | B10.A | NA | B10.A |
AKR/J | 100 | 100 | 100 | 100 | 100 | 100 |
B10.BR | 100 | 100 | 100 | 100 | 100 | 100 |
BALB.K | 100 | 100 | 100 | 100 | 100 | 100 |
MHC locus-specific PCR amplicons from cDNA templates spanning exons 2-4 (class I genes) and exons 2-3 (class II genes) were directly sequenced in both forward and reverse directions. Shown is percent nucleotide sequence homology for each mouse strain with a GenBank sequence at each locus. Reference cDNA sequences were not available for H2-Aβb, H2-Eαb, and H2-Eαk; H2-Eα is not expressed in H2b haplotype strains. Percent homology at the H2-Aβb and H2-Eαk loci represents interstrain comparison of mice used in this study. NA, not available.