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. 2003 Sep 17;100(20):11589–11593. doi: 10.1073/pnas.2034927100

Fig. 3.

Fig. 3.

Calcium flux and MAPK phosphorylation in response to CysLTR ligation in hMCs. (A) Complete blockade of LTC4-induced calcium flux (at 10–7 M) in IL-4-primed hMCs by MK571 (10–6 M). hMCs were primed for 5 days with recombinant IL-4 in the presence of SCF, loaded with fura 2-AM dye, and stimulated with LTC4. Results were identical in a two additional experiments with cells from different donors and replicated earlier data (19). (B) MAPK phosphorylation in IL-4-primed hMCs stimulated for 5 min (ERK blots) or 30 min (p38 blots) with LTC4 (10–7 M) with or without pretreatment with MK571 (10–6 M). Results are from one experiment representative of two performed.