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. 2003 Sep 12;100(20):11618–11623. doi: 10.1073/pnas.1534804100

Fig. 1.

Fig. 1.

Effect of PMA on MUC5AC expression. NCI-H292 cells were treated with vehicle or PMA at various concentrations and times. Total RNA was prepared and subjected to RT-PCR; Rig/S15 was used as an internal marker. (A) Effect of increasing concentrations of PMA given for 6 h on RT-PCR-amplified MUC5AC mRNA expression. (B) Effect of PMA (10 ng/ml) given for various times on RT-PCR-amplified MUC5AC mRNA expression. (C) Effect of increasing concentrations of PMA on total MUC5AC protein production during 24 h by ELISA. (D) Effect of PMA (10 ng/ml) given for various times on total MUC5AC protein production by ELISA. Mucins were measured in the cell lysate (shaded areas) and in the supernatant (unshaded areas). Data in C and D are expressed as mean ± SD. *, P < 0.01, compared with vehicle alone.