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. 2003 Sep 19;100(20):11624–11629. doi: 10.1073/pnas.1931483100

Fig. 2.

Fig. 2.

Akt regulates Foxa-2 via a conserved Akt phosphorylation site. (A) Sequence alignment of orthologous and paralogous members of the Foxa family. (B) HepG2 cells were transfected with expression vectors for Foxa-2, Foxa-2T156A, or Foxa-2R153K together with Akt in varying concentrations. p6xCdx-TkLuc was used as reporter gene. (C) HepG2 cells were transfected with expression vectors for Foxa-2, Foxa-2T156A, or Foxa-2R153K together with Akt at indicated concentrations. pPEPCK-Luc was used as a reporter gene. In all experiments luciferase activity was normalized to β-galactosidase activity. All vectors were transfected at 125 ng, unless stated otherwise. Values are mean of six independent experiments ± SD. *, P ≤ 0.01; **, P ≤ 0.001.