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Akt interacts with Foxa-2 via the conserved Akt phosphorylation site. HEK293 cells were transfected with expression vectors for Foxa-2, Foxa-2T156A, or Foxa-2R153K together with HA-Akt (5 μg). HA-Akt was precipitated by using anti-HA-antibody (Left). Foxa-2 was precipitated by using an anti-Foxa-2 antibody (Right). Cell lysates (Lys.) and precipitates (IP) were separated by SDS/PAGE and analyzed for Foxa-2 or Akt by Western blotting.
