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. 2003 Sep 19;100(20):11630–11635. doi: 10.1073/pnas.2035020100

Fig. 3.

Fig. 3.

BCR-ABL up-regulates and tyrosine phosphorylates IL-3Rβc/β chains independent of IL-3 expression. (A) IL-3-dependent and cytokine-independent IL-3Rβc/β chains activated signaling pathways (reviewed in ref. 33). PROL., proliferation; ANTI-APOP., antiapoptosis; DIFF., differentiation; STIM, stimulation. (B) IL-3Rα protein is not up-regulated in ES-derived hematopoietic progenitors on BCR-ABL induction. (C) IL-3 mRNA expression cannot be detected in ES-derived hematopoietic progenitors in the presence or absence of BCR-ABL induction by RT-PCR analyses. WEHI-3 RNA was used as a control for IL-3 RT-PCR. Three sets of BCR-ABL-expressing and control ES-derived hematopoietic progenitors gave similar results. (D) BCR-ABL leads to IL-3Rβc/β chain up-regulation and activation independent of IL-3 production. BaF3 and ES-derived hematopoietic progenitors were stimulated with 0.1, 1.0, and 10 ng/ml IL-3 for 10 min as positive controls with similar results, and NIH 3T3 cells were used as a negative control for IL-3Rβc/β chain expression and tyrosine phosphorylation. NIH, NIH 3T3.