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. 2003 Sep 16;100(20):11636–11641. doi: 10.1073/pnas.1934692100

Fig. 1.

Fig. 1.

Effects of Oligo AS alone or in combination with HCPT or paclitaxel on apoptosis in prostate cancer cells. The cells were transfected with Oligo AS or ASM at various concentrations for 12 h (A, LNCaP) or 24 h (B, DU145; C, PC3). In combination treatment with HCPT, the cells were transfected with 50 nM Oligo AS or ASM for 12 h (D, LNCaP) or 24 h (E, DU145; F, PC3) and then exposed to HCPT for an additional 12 h (D and E) or 24 h (F). In combination treatment with paclitaxel, the cells were transfected with 50 nM Oligo AS or ASM for 12 h (G, LNCaP) or 24 h (H, DU145; I, PC3) and then exposed to paclitaxel for an additional 12 h (G) or 36 h (H and I). Cells that stained positive for annexin V-FITC (early apoptosis) and positive for FITC and propidium iodide (late apoptosis) were counted. Relative levels of apoptotic indices were expressed as percentage of Lipofectin control. (D–I) Filled circles, Lipofectin control; filled squares, Oligo ASM 50 nM; filled triangles, Oligo AS 50 nM.