Table 1. Evolution of 1a/2a intertypic p7 chimera in chimpanzee 1625.

	p7				NS2	NS3		NS4B
	795		796		918	1202	1531	1745	1763	1906
H77C	P		L		L	G	T	V	K	V
J6CF	S		F		L	D	T	D	K	V
p7-J6/H77-tail	S		F		L	G	T	V	K	V
Weeks p.i.
2	S	90S/1P	F	91F
2.5	S	32S	L/f	20L/12F
3	S	19S	L/f	15L/3F/1I
3.5	S	16S	L/f	9L/7F
4	S	34S	L/f	29L/5F
4.5	S	23S/1P	L	23L/1F
5	S	19S	L	19L
5.5	S		L
6	S		L
6.5	S	34S/2P	L	36L	L/i	G	T	V	K/r	V
7	S		L
7.5	S		L
8	S	21S	L	21L
8.5	S		L
9	S		L
9.5	S	24S	L	24L
10	S		L
10.5	S		L
11	S/p	31S/16P	L	47L	F	E	A	V	R/k	V
11.5	S/p		L
12	S/p	15S/3P	L	18L
12.5	S/p		L
13	S/p		L
13.5
14	S/p		L
14.5	S/p	21S/3P	L	24L	F	E	A	V/a	R/k	A
15	S/p		L
15.5	S		L
16	S		L
16.5	S		L
17	S/p		L
17.5	S/p		L
18	S/p		L
18.5	S/p	22S/2P	L	24L	F	E	A/t	A/v	R/k	A
19	S/p		L
19.5	S		L
20	S	21S	L	21L

The consensus sequence of the ORF of viruses recovered from serum at weeks 6.5, 11, 14.5, and 18.5 posttransfection was determined. Only amino acid changes are given. The corresponding amino acids and their position in the H77C (infectious 1a clone) polyprotein are shown in the first row, followed by the sequence of J6CF (infectious 2a clone) and the viable p7 chimera p7-J6/H77-tail in the second and third rows, respectively. The differences from the p7-J6/H77-tail plasmid sequence in sequences recovered from chimpanzee 1625 are indicated below: dominant sequences are shown in capital letters, and minor sequences are shown in lowercase letters. For sequential analysis of the p7 positions with heterogeneity, the consensus sequence of a subgenomic region (amino acids 702-812) was determined. Clonal analysis of p7 also was performed, and the results are indicated to the right of positions with changes observed in the consensus sequence. Note that at position 795 the consensus sequence did not change, but significant quasispecies were observed. p.i., Postinoculation.