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Analysis of sAPP isoforms secreted by WT-BACE- and GPI-BACE-expressing cells. Media were collected from untransfected SH-SY5Y cells or cells stably expressing WT-BACE or GPI-BACE as described in Fig. 5. (A) Detection of KPI-containing APP isoforms by using an anti-KPI antibody. (B) Media samples were treated with neuraminidase and O-glycosidase as described in Experimental Procedures. Treated and untreated samples were analyzed by SDS/PAGE and immunoblotting with the G26 antibody.
