Fig. 2.

Geldanamycin inhibits RPS2-dependent HR and resistance. (A) The HR test by trypan blue staining. The right half of the leaves from 6-week-old Arabidopsis Col-0 plants (containing both RPM1 and RPS2) were infiltrated with Pst DC3000 strains (1 × 10⁷ cfu/ml) containing vector only, or clones expressing avrRpm1 or avrRpt2. GDA (10μM) or mock solutions lacking GDA were infiltrated together with the bacterial pathogens. Leaves were stained with trypan blue 8 or 20 hpi. Fractions indicate numbers of leaves exhibiting HR and total number of leaves tested. (B) Bacterial growth analysis of Pst DC3000 strains (1 × 10⁵ cfu/ml) containing vector only, or clones expressing avrRpm1 or avrRpt2, inoculated into Arabidopsis Col-0, together with GDA (pink line) or solution lacking GDA (blue line). These experiments were performed three times with similar results.